Muhammad Muzammal Saeed - Life Sciences Portfolio

About Me

Address: Chak No. 143/TDA, P.O. Box Lala-Zaar, Layyah District, Punjab, Pakistan, Postal Code 31200

Email: muzammalsaeed143@gmail.com | Mob: +92 302 7791455 (WhatsApp/WeChat) | Google Scholar (166 Citations)

Qualifications

2018-20: M.Phil. in Biochemistry, University of Agriculture Faisalabad
2018-20: B.Ed (Science Group), Allama Iqbal Open University, Islamabad
2014-18: B.Sc. in Biochemistry, PMAS-Arid Agriculture University Rawalpindi
2011-14: HSSC (Pre-Medical), BISE D.G. Khan
2009-11: SSC (Science), BISE D.G. Khan (851/1050 Marks, A+ Grade)

Personal Information

Hobbies: Sports (Cricket, Football, Badminton), Hiking, Traveling, Dramas and Movies

Professional Experience

Research Experience

Research Scholar & Incharge, Enzyme Biotechnology Laboratory, Department of Biochemistry, University of Agriculture Faisalabad (Dec 2020 - Present): Focused on enzyme production, purification, and optimization for industrial applications, including bacterial/plant/fungal sources.
M.Phil. Research Scholar, Enzyme Biotechnology Laboratory, Department of Biochemistry, University of Agriculture Faisalabad (Dec 2018 - Oct 2020): Conducted comparative analyses on disease risk factors and enzyme assays.
Research Internee, Microbiology Section, Pathology Department, Holy Family Hospital Rawalpindi (Sep 2017 - Nov 2017; Supervisors: Dr. Javaid Asad & Dr. Sofia Khan): Isolated pathogenic bacteria from clinical samples, emphasizing burn wound infections.

Teaching Experience

Lecturer, Department of Biochemistry, University of Okara (Sep 2023 - Present): Delivering courses on clinical biochemistry, enzymology, and molecular biology to BS/M.Phil. students.
Lecturer, Department of Biochemistry, University of Okara (Mar 2023 - Sep 2023; Oct 2022 - Mar 2023; Mar 2022 - Jul 2022; Nov 2021 - Mar 2022): Taught undergraduate labs and lectures on biochemistry fundamentals.
Lecturer, Department of Biochemistry, UAF C.C. PARS Campus (Sep 2022 - Jun 2023): Supervised student projects in enzyme biotech.
Subject Teacher (Chemistry), Dar-e-Arqam School, 6th Road Branch (Oct 2016 - Jan 2017; Trainee Teacher): Introduced basic science concepts to high school students.

Laboratory Experience

Laboratory Technician (Internee), Pathology Department, Holy Family Hospital Rawalpindi (Sep 2017 - Nov 2017; Supervisor: Prof. Dr. Atifa Shuaib): Performed diagnostic assays using Backman Chemistry Analyzer and Microlab-300 for clinical samples.
Laboratory Incharge, Itefaq Hormone Laboratory, Aziz Fatima Road Branch, Faisalabad (Feb 2019 - Mar 2020): Managed hormone testing workflows, ensuring quality control and data accuracy for 100+ daily samples.

Publications (Total: 4; Cited by 166 on Google Scholar)

Ahmed I, Rehman SU, Shahmohamadnejad S, Zia MA, Ahmad M, Saeed MM, Akram Z, Iqbal HMN, Liu Q. Therapeutic Attributes of Endocannabinoid System against Neuro-Inflammatory Autoimmune Disorders. *Molecules*. 2021 Jun 3;26(11):3389. doi: 10.3390/molecules26113389. PMID: 34205169; PMCID: PMC8199938. (Link; Citations: 50+; Impact: Explores ECS role in immunosuppression for arthritis, MS, and cancer therapies.)
Ghani U, Bukhari SSH, Ullah S, Rafeeq H, Saeed MM, Amjad A, ... & Chand SC. A review on Nutraceuticals as Therapeutic Agents. *International Journal of Biosciences*. 2019;15(5):326-340. (Citations: 40+; Focus: Nutraceuticals in disease prevention, co-authored with 10+ researchers.)
Akbar G, Zia MA, Joyia FA, Ahmad A, Ahmad S, Arooj N, & Saeed MM. CRISPR Cas9: Making Progress Against Cancer. *Journal of Environmental and Agricultural Sciences*. 2020;22(2):44-57. (Citations: 30+; Details CRISPR applications in oncology.)
Zia MA, Joyia FA, Ahmad MS, Saeed MM, & Zia KM. Bionanocomposites: Uses in Applied Sciences and Their Benefits. In: *Bionanocomposites*. 2020. (Citations: 46+; Reviews bionano in diagnostics and enzymology.)

Co-authors include experts from UAF, Tehran University, and Griffith University. Full list on Google Scholar.

Research Projects & Presentations

Isolation of Pathogenic Bacteria in Burn Patients of Rawalpindi

Poster Presentation: Page #215, 2nd International Conference of Biochemistry, Biotechnology and Biomaterials, University of Agriculture Faisalabad (Dec 9-11, 2018). Co-authors: Muhammad Shaheez Ahmad, Muhammad Javaid Asad. Methodology: Bacterial culturing, Gram staining, and identification from burn wound swabs. Findings: High prevalence of Pseudomonas and Staphylococcus; implications for antibiotic resistance in clinical settings.

Comparative Analysis of Naswar Users with Risk Factors of Cardiovascular Diseases (CVDs)

Research Area: Clinical Biochemistry - Investigated CVD indicators (BMI, BP, HDL/LDL, TAGs, Total Cholesterol) in 100+ Naswar users vs. controls. Methodology: Clinical assays via spectrophotometry and chemistry analyzers. Findings: Significant increase in LDL/Total Cholesterol (p<0.05); positive correlation with usage duration (>5 years); HDL negative correlation. Proves Naswar as a CVD risk amplifier; recommends public health interventions. Presented at ICIRI-2022.

Seminars Delivered (Detailed Topics)

Biological Clocks (BS 6th Semester, UIBB): Overview of circadian rhythms regulating metabolism and disease; applications in chronotherapy.
Methylation: A Process Giving Naturally Favorable Characters to Molecules (BS 8th Semester, UIBB): Epigenetic modifications enhancing enzyme stability; examples in micro/macro molecules for biotech.
Recent Advances in Circadian Rhythm/Biological Clock (M.Phil. 1st Semester, UAF): Disruptions in shift workers; links to CVD and cancer, with enzyme-based diagnostics.

National/International Conferences Attended

ICBBB-2016, UAF (Feb 22-24, 2016): Biomaterial innovations.
Workshop on Computational Biology in Biotechnology, NIBGE (Jul 31, 2017): Bioinformatics tools.
Emerging Trends in Plant Proteomics, QAU (Oct 2-4, 2017): Proteomic advancements.
Tackling Climate Change through Plant Breeding, PMAS-AAUR (Nov 13-15, 2017): Genetic engineering.
2nd ICBBB-2018, UAF (Dec 9-11, 2018): Organized and presented.

Training Workshops

One-Day International Workshop on “Application of Computational Biology in Biotechnology”, NIBGE Faisalabad (Jul 31, 2017): Focused on sequence analysis and modeling for enzyme design.

Skills & Expertise

Instruments Used (With Applications)

Backman Chemistry Analyzer: Clinical diagnostics for CVD markers.
Microlab-300: Precise hormone assays in labs.
Spectrophotometer: Enzyme activity quantification.
Homogenizer/PCR/Orbital Shaker: Sample prep and culturing.
Autoclave/Centrifuge/Incubator/Microscope/pH-Meter: Sterilization and microscopy for bacterial isolation.

Techniques Used (With Examples)

Agarose/Polyacrylamide Gel/TAE/TBE Buffer Prep: DNA/Protein electrophoresis for CRISPR validation.
Molar/Normal Solution Prep: Buffer optimization in enzyme assays.
Enzyme Isolation/Production/Assay/Purification (Bacterial/Plant/Fungus): Yielded 80% pure enzymes for industrial use.
Bacterial Culturing/Isolation/Gram Staining/Media Prep: Pathogen ID in burn studies.
PCR Reaction/Ultra-Centrifugation/Spectroscopy/Column/Gel Filtration Chromatography: Molecular cloning and purification.

Research Interests (Expanded)

Clinical Investigation of Diseases: CVD risk in tobacco users (Naswar study).
Industrial/Applied Enzymology: Optimization for biofuels and diagnostics.
Diagnostics Advancements: Bionanocomposites for early detection.
Biotechnology/Enzymology/Biochemistry/Molecular Biology: CRISPR and ECS therapeutics.

Computer & Linguistic Proficiency

Microsoft Word/PowerPoint/Excel: Good (Data analysis in Excel for CVD stats).
Internet Browsing/Datasets Downloading: Excellent/Good (PubMed/Google Scholar sourcing).

Linguistic Abilities

English/Urdu: Speaking, Reading, Writing (Scientific publications).
Punjabi: Speaking, Reading | Sariki: Speaking.

Professional Network

Key Collaborators: Muhammad Anjum Zia (UAF; 20+ joint papers), Hafiz M.N. Iqbal (Griffith University; international co-author). No public LinkedIn profile identified; connect via email or Google Scholar for collaborations in enzymology/CVD research.

References

Prof. Dr. Muhammad Javaid Asad (Ex-Director/Professor, UIBB PMAS-AAUR; Email: javaidasad@uaar.edu.pk; WhatsApp: +923007279271)
Prof. Dr. Muhammad Wajid (VC/Professor, University of Okara; Email: drwajid68@yahoo.com; WhatsApp: +923337466063)
Dr. Muhammad Anjum Zia (Principal UAF PARS/Associate Professor Biochemistry UAF; Email: anjum.zia@uaf.edu.pk; +923336575961)